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synthesis of the osmolyte glucosylglycerol (gg) in the marine cyanobacterium synechococcus sp. strain pcc 7002 was characterized. the ggps gene, which encodes the key enzyme (gg-phosphate synthase [ggps]) in gg biosynthesis, was cloned by using pcr. a 2,030-bp dna sequence which contained one open reading frame (orf) was obtained. the protein deduced from this orf exhibited 85% similarity to
The ggpS Gene from Synechocystis sp. Strain PCC 6803
a salt-sensitive mutant of synechocystis sp. strain pcc 6803 defective in the synthesis of the compatible solute glucosylglycerol (gg) was used to search for the gene encoding gg-phosphate synthase (ggps), the key enzyme in gg synthesis. cloning and sequencing of the mutated region and the corresponding wild-type region revealed that a deletion of about 13 kb occurred in the genome of mutant 11.
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